The In Ovo Delivery of CpG Oligonucleotides Protects against Infectious Bronchitis with the Recruitment of Immune Cells into the Respiratory Tract of Chickens
Identifieur interne : 000688 ( Main/Exploration ); précédent : 000687; suivant : 000689The In Ovo Delivery of CpG Oligonucleotides Protects against Infectious Bronchitis with the Recruitment of Immune Cells into the Respiratory Tract of Chickens
Auteurs : Upasama De Silva Senapathi ; Mohamed Sarjoon Abdul-Cader ; Aruna Amarasinghe ; Guido Van Marle ; Markus Czub ; Susantha Gomis ; Mohamed Faizal Abdul-CareemSource :
- Viruses [ 1999-4915 ] ; 2018.
Abstract
The in ovo delivery of cytosine-guanosine (CpG) oligodeoxynucleotides (ODNs) protects chickens against many bacterial and viral infections, by activating the toll-like receptor (TLR)21 signaling pathway. Although the delivery of CpG ODNs in ovo at embryo day (ED) 18 has been shown to reduce infectious bronchitis virus (IBV) loads in embryonic chicken lungs pre-hatch, whether in ovo delivered CpG ODNs are capable of protecting chickens against a post-hatch challenge is unknown. Thus, our objectives were to determine the protective effect of the in ovo delivery of CpG ODNs at ED 18 against IBV infection encountered post-hatch and, then, to investigate the mechanisms of protection. We found significantly higher survival rates and reduced IBV infection in the chickens following the pre-treatment of the ED 18 eggs with CpG ODNs. At 3 days post infection (dpi), we found an increased recruitment of macrophages, cluster of differentiation (CD)8α+ and CD4+ T lymphocytes, and an up-regulation of interferon (IFN)-γ mRNA in the respiratory tract of the chickens. Overall, it may be inferred that CpG ODNs, when delivered in ovo, provide protection against IBV infection induced morbidity and mortality with an enhanced immune response.
Url:
DOI: 10.3390/v10110635
PubMed: 30445707
PubMed Central: 6266937
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000737
- to stream Pmc, to step Curation: 000737
- to stream Pmc, to step Checkpoint: 000108
- to stream Ncbi, to step Merge: 000736
- to stream Ncbi, to step Curation: 000736
- to stream Ncbi, to step Checkpoint: 000736
- to stream Main, to step Merge: 000690
- to stream Main, to step Curation: 000688
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The In Ovo Delivery of CpG Oligonucleotides Protects against Infectious Bronchitis with the Recruitment of Immune Cells into the Respiratory Tract of Chickens</title><author><name sortKey="De Silva Senapathi, Upasama" sort="De Silva Senapathi, Upasama" uniqKey="De Silva Senapathi U" first="Upasama" last="De Silva Senapathi">Upasama De Silva Senapathi</name><affiliation><nlm:aff id="af1-viruses-10-00635">Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada;<email>yaseshwari.desilvase@ucalgary.ca</email> (U.D.S.S.);<email>mohamedsarjoon.moham@ucalgary.ca</email> (M.S.A.-C.);<email>arunavetlk@gmail.com</email> (A.A.);<email>vanmarle@ucalgary.ca</email> (G.v.M.);<email>m.czub@ucalgary.ca</email> (M.C.)</nlm:aff></affiliation></author><author><name sortKey="Abdul Cader, Mohamed Sarjoon" sort="Abdul Cader, Mohamed Sarjoon" uniqKey="Abdul Cader M" first="Mohamed Sarjoon" last="Abdul-Cader">Mohamed Sarjoon Abdul-Cader</name><affiliation><nlm:aff id="af1-viruses-10-00635">Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada;<email>yaseshwari.desilvase@ucalgary.ca</email> (U.D.S.S.);<email>mohamedsarjoon.moham@ucalgary.ca</email> (M.S.A.-C.);<email>arunavetlk@gmail.com</email> (A.A.);<email>vanmarle@ucalgary.ca</email> (G.v.M.);<email>m.czub@ucalgary.ca</email> (M.C.)</nlm:aff></affiliation></author><author><name sortKey="Amarasinghe, Aruna" sort="Amarasinghe, Aruna" uniqKey="Amarasinghe A" first="Aruna" last="Amarasinghe">Aruna Amarasinghe</name><affiliation><nlm:aff id="af1-viruses-10-00635">Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada;<email>yaseshwari.desilvase@ucalgary.ca</email> (U.D.S.S.);<email>mohamedsarjoon.moham@ucalgary.ca</email> (M.S.A.-C.);<email>arunavetlk@gmail.com</email> (A.A.);<email>vanmarle@ucalgary.ca</email> (G.v.M.);<email>m.czub@ucalgary.ca</email> (M.C.)</nlm:aff></affiliation></author><author><name sortKey="Van Marle, Guido" sort="Van Marle, Guido" uniqKey="Van Marle G" first="Guido" last="Van Marle">Guido Van Marle</name><affiliation><nlm:aff id="af1-viruses-10-00635">Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada;<email>yaseshwari.desilvase@ucalgary.ca</email> (U.D.S.S.);<email>mohamedsarjoon.moham@ucalgary.ca</email> (M.S.A.-C.);<email>arunavetlk@gmail.com</email> (A.A.);<email>vanmarle@ucalgary.ca</email> (G.v.M.);<email>m.czub@ucalgary.ca</email> (M.C.)</nlm:aff></affiliation></author><author><name sortKey="Czub, Markus" sort="Czub, Markus" uniqKey="Czub M" first="Markus" last="Czub">Markus Czub</name><affiliation><nlm:aff id="af1-viruses-10-00635">Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada;<email>yaseshwari.desilvase@ucalgary.ca</email> (U.D.S.S.);<email>mohamedsarjoon.moham@ucalgary.ca</email> (M.S.A.-C.);<email>arunavetlk@gmail.com</email> (A.A.);<email>vanmarle@ucalgary.ca</email> (G.v.M.);<email>m.czub@ucalgary.ca</email> (M.C.)</nlm:aff></affiliation></author><author><name sortKey="Gomis, Susantha" sort="Gomis, Susantha" uniqKey="Gomis S" first="Susantha" last="Gomis">Susantha Gomis</name><affiliation><nlm:aff id="af2-viruses-10-00635">Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B5, Canada;<email>smg127@mail.usask.ca</email></nlm:aff></affiliation></author><author><name sortKey="Abdul Careem, Mohamed Faizal" sort="Abdul Careem, Mohamed Faizal" uniqKey="Abdul Careem M" first="Mohamed Faizal" last="Abdul-Careem">Mohamed Faizal Abdul-Careem</name><affiliation><nlm:aff id="af1-viruses-10-00635">Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada;<email>yaseshwari.desilvase@ucalgary.ca</email> (U.D.S.S.);<email>mohamedsarjoon.moham@ucalgary.ca</email> (M.S.A.-C.);<email>arunavetlk@gmail.com</email> (A.A.);<email>vanmarle@ucalgary.ca</email> (G.v.M.);<email>m.czub@ucalgary.ca</email> (M.C.)</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">30445707</idno><idno type="pmc">6266937</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266937</idno><idno type="RBID">PMC:6266937</idno><idno type="doi">10.3390/v10110635</idno><date when="2018">2018</date><idno type="wicri:Area/Pmc/Corpus">000737</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000737</idno><idno type="wicri:Area/Pmc/Curation">000737</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000737</idno><idno type="wicri:Area/Pmc/Checkpoint">000108</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000108</idno><idno type="wicri:Area/Ncbi/Merge">000736</idno><idno type="wicri:Area/Ncbi/Curation">000736</idno><idno type="wicri:Area/Ncbi/Checkpoint">000736</idno><idno type="wicri:Area/Main/Merge">000690</idno><idno type="wicri:Area/Main/Curation">000688</idno><idno type="wicri:Area/Main/Exploration">000688</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The In Ovo Delivery of CpG Oligonucleotides Protects against Infectious Bronchitis with the Recruitment of Immune Cells into the Respiratory Tract of Chickens</title><author><name sortKey="De Silva Senapathi, Upasama" sort="De Silva Senapathi, Upasama" uniqKey="De Silva Senapathi U" first="Upasama" last="De Silva Senapathi">Upasama De Silva Senapathi</name><affiliation><nlm:aff id="af1-viruses-10-00635">Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada;<email>yaseshwari.desilvase@ucalgary.ca</email> (U.D.S.S.);<email>mohamedsarjoon.moham@ucalgary.ca</email> (M.S.A.-C.);<email>arunavetlk@gmail.com</email> (A.A.);<email>vanmarle@ucalgary.ca</email> (G.v.M.);<email>m.czub@ucalgary.ca</email> (M.C.)</nlm:aff></affiliation></author><author><name sortKey="Abdul Cader, Mohamed Sarjoon" sort="Abdul Cader, Mohamed Sarjoon" uniqKey="Abdul Cader M" first="Mohamed Sarjoon" last="Abdul-Cader">Mohamed Sarjoon Abdul-Cader</name><affiliation><nlm:aff id="af1-viruses-10-00635">Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada;<email>yaseshwari.desilvase@ucalgary.ca</email> (U.D.S.S.);<email>mohamedsarjoon.moham@ucalgary.ca</email> (M.S.A.-C.);<email>arunavetlk@gmail.com</email> (A.A.);<email>vanmarle@ucalgary.ca</email> (G.v.M.);<email>m.czub@ucalgary.ca</email> (M.C.)</nlm:aff></affiliation></author><author><name sortKey="Amarasinghe, Aruna" sort="Amarasinghe, Aruna" uniqKey="Amarasinghe A" first="Aruna" last="Amarasinghe">Aruna Amarasinghe</name><affiliation><nlm:aff id="af1-viruses-10-00635">Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada;<email>yaseshwari.desilvase@ucalgary.ca</email> (U.D.S.S.);<email>mohamedsarjoon.moham@ucalgary.ca</email> (M.S.A.-C.);<email>arunavetlk@gmail.com</email> (A.A.);<email>vanmarle@ucalgary.ca</email> (G.v.M.);<email>m.czub@ucalgary.ca</email> (M.C.)</nlm:aff></affiliation></author><author><name sortKey="Van Marle, Guido" sort="Van Marle, Guido" uniqKey="Van Marle G" first="Guido" last="Van Marle">Guido Van Marle</name><affiliation><nlm:aff id="af1-viruses-10-00635">Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada;<email>yaseshwari.desilvase@ucalgary.ca</email> (U.D.S.S.);<email>mohamedsarjoon.moham@ucalgary.ca</email> (M.S.A.-C.);<email>arunavetlk@gmail.com</email> (A.A.);<email>vanmarle@ucalgary.ca</email> (G.v.M.);<email>m.czub@ucalgary.ca</email> (M.C.)</nlm:aff></affiliation></author><author><name sortKey="Czub, Markus" sort="Czub, Markus" uniqKey="Czub M" first="Markus" last="Czub">Markus Czub</name><affiliation><nlm:aff id="af1-viruses-10-00635">Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada;<email>yaseshwari.desilvase@ucalgary.ca</email> (U.D.S.S.);<email>mohamedsarjoon.moham@ucalgary.ca</email> (M.S.A.-C.);<email>arunavetlk@gmail.com</email> (A.A.);<email>vanmarle@ucalgary.ca</email> (G.v.M.);<email>m.czub@ucalgary.ca</email> (M.C.)</nlm:aff></affiliation></author><author><name sortKey="Gomis, Susantha" sort="Gomis, Susantha" uniqKey="Gomis S" first="Susantha" last="Gomis">Susantha Gomis</name><affiliation><nlm:aff id="af2-viruses-10-00635">Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B5, Canada;<email>smg127@mail.usask.ca</email></nlm:aff></affiliation></author><author><name sortKey="Abdul Careem, Mohamed Faizal" sort="Abdul Careem, Mohamed Faizal" uniqKey="Abdul Careem M" first="Mohamed Faizal" last="Abdul-Careem">Mohamed Faizal Abdul-Careem</name><affiliation><nlm:aff id="af1-viruses-10-00635">Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada;<email>yaseshwari.desilvase@ucalgary.ca</email> (U.D.S.S.);<email>mohamedsarjoon.moham@ucalgary.ca</email> (M.S.A.-C.);<email>arunavetlk@gmail.com</email> (A.A.);<email>vanmarle@ucalgary.ca</email> (G.v.M.);<email>m.czub@ucalgary.ca</email> (M.C.)</nlm:aff></affiliation></author></analytic><series><title level="j">Viruses</title><idno type="eISSN">1999-4915</idno><imprint><date when="2018">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The in ovo delivery of cytosine-guanosine (CpG) oligodeoxynucleotides (ODNs) protects chickens against many bacterial and viral infections, by activating the toll-like receptor (TLR)21 signaling pathway. Although the delivery of CpG ODNs in ovo at embryo day (ED) 18 has been shown to reduce infectious bronchitis virus (IBV) loads in embryonic chicken lungs pre-hatch, whether in ovo delivered CpG ODNs are capable of protecting chickens against a post-hatch challenge is unknown. Thus, our objectives were to determine the protective effect of the in ovo delivery of CpG ODNs at ED 18 against IBV infection encountered post-hatch and, then, to investigate the mechanisms of protection. We found significantly higher survival rates and reduced IBV infection in the chickens following the pre-treatment of the ED 18 eggs with CpG ODNs. At 3 days post infection (dpi), we found an increased recruitment of macrophages, cluster of differentiation (CD)8α+ and CD4+ T lymphocytes, and an up-regulation of interferon (IFN)-γ mRNA in the respiratory tract of the chickens. Overall, it may be inferred that CpG ODNs, when delivered in ovo, provide protection against IBV infection induced morbidity and mortality with an enhanced immune response.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Hitchner, S" uniqKey="Hitchner S">S. Hitchner</name></author><author><name sortKey="Winterfield, R" uniqKey="Winterfield R">R. Winterfield</name></author><author><name sortKey="Appleton, G" uniqKey="Appleton G">G. Appleton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kameka, A M" uniqKey="Kameka A">A.M. Kameka</name></author><author><name sortKey="Haddadi, S" uniqKey="Haddadi S">S. Haddadi</name></author><author><name sortKey="Kim, D S" uniqKey="Kim D">D.S. Kim</name></author><author><name sortKey="Cork, S C" uniqKey="Cork S">S.C. Cork</name></author><author><name sortKey="Abdul Careem, M F" uniqKey="Abdul Careem M">M.F. Abdul-Careem</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ignjatovic, J" uniqKey="Ignjatovic J">J. Ignjatovic</name></author><author><name sortKey="Ashton, D" uniqKey="Ashton D">D. Ashton</name></author><author><name sortKey="Reece, R" uniqKey="Reece R">R. Reece</name></author><author><name sortKey="Scott, P" uniqKey="Scott P">P. Scott</name></author><author><name sortKey="Hooper, P" uniqKey="Hooper P">P. Hooper</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, S" uniqKey="Liu S">S. Liu</name></author><author><name sortKey="Chen, J" uniqKey="Chen J">J. Chen</name></author><author><name sortKey="Chen, J" uniqKey="Chen J">J. Chen</name></author><author><name sortKey="Kong, X" uniqKey="Kong X">X. Kong</name></author><author><name sortKey="Shao, Y" uniqKey="Shao Y">Y. Shao</name></author><author><name sortKey="Han, Z" uniqKey="Han Z">Z. Han</name></author><author><name sortKey="Feng, L" uniqKey="Feng L">L. Feng</name></author><author><name sortKey="Cai, X" uniqKey="Cai X">X. Cai</name></author><author><name sortKey="Gu, S" uniqKey="Gu S">S. Gu</name></author><author><name sortKey="Liu, M" uniqKey="Liu M">M. Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, B" uniqKey="Chen B">B. Chen</name></author><author><name sortKey="Hosi, S" uniqKey="Hosi S">S. Hosi</name></author><author><name sortKey="Nunoya, T" uniqKey="Nunoya T">T. Nunoya</name></author><author><name sortKey="Itakura, C" uniqKey="Itakura C">C. Itakura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, B" uniqKey="Chen B">B. Chen</name></author><author><name sortKey="Itakura, C" uniqKey="Itakura C">C. Itakura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Raj, G D" uniqKey="Raj G">G.D. Raj</name></author><author><name sortKey="Jones, R" uniqKey="Jones R">R. Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fabricant, J" uniqKey="Fabricant J">J. Fabricant</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bande, F" uniqKey="Bande F">F. Bande</name></author><author><name sortKey="Arshad, S S" uniqKey="Arshad S">S.S. Arshad</name></author><author><name sortKey="Omar, A R" uniqKey="Omar A">A.R. Omar</name></author><author><name sortKey="Hair Bejo, M" uniqKey="Hair Bejo M">M. Hair-Bejo</name></author><author><name sortKey="Mahmuda, A" uniqKey="Mahmuda A">A. Mahmuda</name></author><author><name sortKey="Nair, V" uniqKey="Nair V">V. Nair</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Banat, G R" uniqKey="Banat G">G.R. Banat</name></author><author><name sortKey="Tkalcic, S" uniqKey="Tkalcic S">S. Tkalcic</name></author><author><name sortKey="Dzielawa, J A" uniqKey="Dzielawa J">J.A. Dzielawa</name></author><author><name sortKey="Jackwood, M W" uniqKey="Jackwood M">M.W. Jackwood</name></author><author><name sortKey="Saggese, M D" uniqKey="Saggese M">M.D. Saggese</name></author><author><name sortKey="Yates, L" uniqKey="Yates L">L. Yates</name></author><author><name sortKey="Kopulos, R" uniqKey="Kopulos R">R. Kopulos</name></author><author><name sortKey="Briles, W" uniqKey="Briles W">W. Briles</name></author><author><name sortKey="Collisson, E W" uniqKey="Collisson E">E.W. Collisson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ignjatovic, J" uniqKey="Ignjatovic J">J. Ignjatovic</name></author><author><name sortKey="Sapats, S" uniqKey="Sapats S">S. Sapats</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chousalkar, K" uniqKey="Chousalkar K">K. Chousalkar</name></author><author><name sortKey="Roberts, J" uniqKey="Roberts J">J. Roberts</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Purchase, H" uniqKey="Purchase H">H. Purchase</name></author><author><name sortKey="Cunningham, C" uniqKey="Cunningham C">C. Cunningham</name></author><author><name sortKey="Burmester, B" uniqKey="Burmester B">B. Burmester</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Aucouturier, J" uniqKey="Aucouturier J">J. Aucouturier</name></author><author><name sortKey="Dupuis, L" uniqKey="Dupuis L">L. Dupuis</name></author><author><name sortKey="Ganne, V" uniqKey="Ganne V">V. Ganne</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cavanagh, D" uniqKey="Cavanagh D">D. Cavanagh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="He, H" uniqKey="He H">H. He</name></author><author><name sortKey="Mackinnon, K M" uniqKey="Mackinnon K">K.M. MacKinnon</name></author><author><name sortKey="Genovese, K J" uniqKey="Genovese K">K.J. Genovese</name></author><author><name sortKey="Kogut, M H" uniqKey="Kogut M">M.H. Kogut</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hemmi, H" uniqKey="Hemmi H">H. Hemmi</name></author><author><name sortKey="Akira, S" uniqKey="Akira S">S. Akira</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gomis, S" uniqKey="Gomis S">S. Gomis</name></author><author><name sortKey="Babiuk, L" uniqKey="Babiuk L">L. Babiuk</name></author><author><name sortKey="Godson, D L" uniqKey="Godson D">D.L. Godson</name></author><author><name sortKey="Allan, B" uniqKey="Allan B">B. Allan</name></author><author><name sortKey="Thrush, T" uniqKey="Thrush T">T. Thrush</name></author><author><name sortKey="Townsend, H" uniqKey="Townsend H">H. Townsend</name></author><author><name sortKey="Willson, P" uniqKey="Willson P">P. Willson</name></author><author><name sortKey="Waters, E" uniqKey="Waters E">E. Waters</name></author><author><name sortKey="Hecker, R" uniqKey="Hecker R">R. Hecker</name></author><author><name sortKey="Potter, A" uniqKey="Potter A">A. Potter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Taghavi, A" uniqKey="Taghavi A">A. Taghavi</name></author><author><name sortKey="Allan, B" uniqKey="Allan B">B. Allan</name></author><author><name sortKey="Mutwiri, G" uniqKey="Mutwiri G">G. Mutwiri</name></author><author><name sortKey="Van Kessel, A" uniqKey="Van Kessel A">A. Van Kessel</name></author><author><name sortKey="Willson, P" uniqKey="Willson P">P. Willson</name></author><author><name sortKey="Babiuk, L" uniqKey="Babiuk L">L. Babiuk</name></author><author><name sortKey="Potter, A" uniqKey="Potter A">A. Potter</name></author><author><name sortKey="Gomis, S" uniqKey="Gomis S">S. Gomis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Abdul Cader, M S" uniqKey="Abdul Cader M">M.S. Abdul-Cader</name></author><author><name sortKey="Ahmed Hassan, H" uniqKey="Ahmed Hassan H">H. Ahmed-Hassan</name></author><author><name sortKey="Amarasinghe, A" uniqKey="Amarasinghe A">A. Amarasinghe</name></author><author><name sortKey="Nagy, E" uniqKey="Nagy E">E. Nagy</name></author><author><name sortKey="Sharif, S" uniqKey="Sharif S">S. Sharif</name></author><author><name sortKey="Abdul Careem, M F" uniqKey="Abdul Careem M">M.F. Abdul-Careem</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thapa, S" uniqKey="Thapa S">S. Thapa</name></author><author><name sortKey="Cader, M S A" uniqKey="Cader M">M.S.A. Cader</name></author><author><name sortKey="Murugananthan, K" uniqKey="Murugananthan K">K. Murugananthan</name></author><author><name sortKey="Nagy, E" uniqKey="Nagy E">E. Nagy</name></author><author><name sortKey="Sharif, S" uniqKey="Sharif S">S. Sharif</name></author><author><name sortKey="Czub, M" uniqKey="Czub M">M. Czub</name></author><author><name sortKey="Abdul Careem, M F" uniqKey="Abdul Careem M">M.F. Abdul-Careem</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Abdul Cader, M S" uniqKey="Abdul Cader M">M.S. Abdul-Cader</name></author><author><name sortKey="Amarasinghe, A" uniqKey="Amarasinghe A">A. Amarasinghe</name></author><author><name sortKey="Palomino Tapia, V" uniqKey="Palomino Tapia V">V. Palomino-Tapia</name></author><author><name sortKey="Ahmed Hassan, H" uniqKey="Ahmed Hassan H">H. Ahmed-Hassan</name></author><author><name sortKey="Bakhtawar, K" uniqKey="Bakhtawar K">K. Bakhtawar</name></author><author><name sortKey="Nagy, E" uniqKey="Nagy E">E. Nagy</name></author><author><name sortKey="Sharif, S" uniqKey="Sharif S">S. Sharif</name></author><author><name sortKey="Gomis, S" uniqKey="Gomis S">S. Gomis</name></author><author><name sortKey="Abdul Careem, M F" uniqKey="Abdul Careem M">M.F. Abdul-Careem</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dar, A" uniqKey="Dar A">A. Dar</name></author><author><name sortKey="Potter, A" uniqKey="Potter A">A. Potter</name></author><author><name sortKey="Tikoo, S" uniqKey="Tikoo S">S. Tikoo</name></author><author><name sortKey="Gerdts, V" uniqKey="Gerdts V">V. Gerdts</name></author><author><name sortKey="Lai, K" uniqKey="Lai K">K. Lai</name></author><author><name sortKey="Babiuk, L A" uniqKey="Babiuk L">L.A. Babiuk</name></author><author><name sortKey="Mutwiri, G" uniqKey="Mutwiri G">G. Mutwiri</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dar, A" uniqKey="Dar A">A. Dar</name></author><author><name sortKey="Tikoo, S" uniqKey="Tikoo S">S. Tikoo</name></author><author><name sortKey="Potter, A" uniqKey="Potter A">A. Potter</name></author><author><name sortKey="Babiuk, L A" uniqKey="Babiuk L">L.A. Babiuk</name></author><author><name sortKey="Townsend, H" uniqKey="Townsend H">H. Townsend</name></author><author><name sortKey="Gerdts, V" uniqKey="Gerdts V">V. Gerdts</name></author><author><name sortKey="Mutwiri, G" uniqKey="Mutwiri G">G. Mutwiri</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chace, J H" uniqKey="Chace J">J.H. Chace</name></author><author><name sortKey="Hooker, N A" uniqKey="Hooker N">N.A. Hooker</name></author><author><name sortKey="Mildenstein, K L" uniqKey="Mildenstein K">K.L. Mildenstein</name></author><author><name sortKey="Krieg, A M" uniqKey="Krieg A">A.M. Krieg</name></author><author><name sortKey="Cowdery, J S" uniqKey="Cowdery J">J.S. Cowdery</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wagner, H" uniqKey="Wagner H">H. Wagner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reed, L J" uniqKey="Reed L">L.J. Reed</name></author><author><name sortKey="Muench, H" uniqKey="Muench H">H. Muench</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Forlenza, M" uniqKey="Forlenza M">M. Forlenza</name></author><author><name sortKey="Kaiser, T" uniqKey="Kaiser T">T. Kaiser</name></author><author><name sortKey="Savelkoul, H F" uniqKey="Savelkoul H">H.F. Savelkoul</name></author><author><name sortKey="Wiegertjes, G F" uniqKey="Wiegertjes G">G.F. Wiegertjes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Abdul Cader, M S" uniqKey="Abdul Cader M">M.S. Abdul-Cader</name></author><author><name sortKey="Palomino Tapia, V" uniqKey="Palomino Tapia V">V. Palomino-Tapia</name></author><author><name sortKey="Amarasinghe, A" uniqKey="Amarasinghe A">A. Amarasinghe</name></author><author><name sortKey="Ahmed Hassan, H" uniqKey="Ahmed Hassan H">H. Ahmed-Hassan</name></author><author><name sortKey="De Silva Senapathi, U" uniqKey="De Silva Senapathi U">U. De Silva Senapathi</name></author><author><name sortKey="Abdul Careem, M F" uniqKey="Abdul Careem M">M.F. Abdul-Careem</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shoda, L K" uniqKey="Shoda L">L.K. Shoda</name></author><author><name sortKey="Kegerreis, K A" uniqKey="Kegerreis K">K.A. Kegerreis</name></author><author><name sortKey="Suarez, C E" uniqKey="Suarez C">C.E. Suarez</name></author><author><name sortKey="Mwangi, W" uniqKey="Mwangi W">W. Mwangi</name></author><author><name sortKey="Knowles, D P" uniqKey="Knowles D">D.P. Knowles</name></author><author><name sortKey="Brown, W C" uniqKey="Brown W">W.C. Brown</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Igarashi, K" uniqKey="Igarashi K">K. Igarashi</name></author><author><name sortKey="Mitsuyama, M" uniqKey="Mitsuyama M">M. Mitsuyama</name></author><author><name sortKey="Muramori, K" uniqKey="Muramori K">K. Muramori</name></author><author><name sortKey="Tsukada, H" uniqKey="Tsukada H">H. Tsukada</name></author><author><name sortKey="Nomoto, K" uniqKey="Nomoto K">K. Nomoto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Davila, E" uniqKey="Davila E">E. Davila</name></author><author><name sortKey="Velez, M G" uniqKey="Velez M">M.G. Velez</name></author><author><name sortKey="Heppelmann, C J" uniqKey="Heppelmann C">C.J. Heppelmann</name></author><author><name sortKey="Celis, E" uniqKey="Celis E">E. Celis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Szabo, S J" uniqKey="Szabo S">S.J. Szabo</name></author><author><name sortKey="Sullivan, B M" uniqKey="Sullivan B">B.M. Sullivan</name></author><author><name sortKey="Stemmann, C" uniqKey="Stemmann C">C. Stemmann</name></author><author><name sortKey="Satoskar, A R" uniqKey="Satoskar A">A.R. Satoskar</name></author><author><name sortKey="Sleckman, B P" uniqKey="Sleckman B">B.P. Sleckman</name></author><author><name sortKey="Glimcher, L H" uniqKey="Glimcher L">L.H. Glimcher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yoshimoto, T" uniqKey="Yoshimoto T">T. Yoshimoto</name></author><author><name sortKey="Takeda, K" uniqKey="Takeda K">K. Takeda</name></author><author><name sortKey="Tanaka, T" uniqKey="Tanaka T">T. Tanaka</name></author><author><name sortKey="Ohkusu, K" uniqKey="Ohkusu K">K. Ohkusu</name></author><author><name sortKey="Kashiwamura, S I" uniqKey="Kashiwamura S">S.-I. Kashiwamura</name></author><author><name sortKey="Okamura, H" uniqKey="Okamura H">H. Okamura</name></author><author><name sortKey="Akira, S" uniqKey="Akira S">S. Akira</name></author><author><name sortKey="Nakanishi, K" uniqKey="Nakanishi K">K. Nakanishi</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Abdul Cader, Mohamed Sarjoon" sort="Abdul Cader, Mohamed Sarjoon" uniqKey="Abdul Cader M" first="Mohamed Sarjoon" last="Abdul-Cader">Mohamed Sarjoon Abdul-Cader</name><name sortKey="Abdul Careem, Mohamed Faizal" sort="Abdul Careem, Mohamed Faizal" uniqKey="Abdul Careem M" first="Mohamed Faizal" last="Abdul-Careem">Mohamed Faizal Abdul-Careem</name><name sortKey="Amarasinghe, Aruna" sort="Amarasinghe, Aruna" uniqKey="Amarasinghe A" first="Aruna" last="Amarasinghe">Aruna Amarasinghe</name><name sortKey="Czub, Markus" sort="Czub, Markus" uniqKey="Czub M" first="Markus" last="Czub">Markus Czub</name><name sortKey="De Silva Senapathi, Upasama" sort="De Silva Senapathi, Upasama" uniqKey="De Silva Senapathi U" first="Upasama" last="De Silva Senapathi">Upasama De Silva Senapathi</name><name sortKey="Gomis, Susantha" sort="Gomis, Susantha" uniqKey="Gomis S" first="Susantha" last="Gomis">Susantha Gomis</name><name sortKey="Van Marle, Guido" sort="Van Marle, Guido" uniqKey="Van Marle G" first="Guido" last="Van Marle">Guido Van Marle</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000688 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000688 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:6266937
|texte= The In Ovo Delivery of CpG Oligonucleotides Protects against Infectious Bronchitis with the Recruitment of Immune Cells into the Respiratory Tract of Chickens
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:30445707" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a CovidV1
| This area was generated with Dilib version V0.6.33. |  |